25-0238-41

antibody from Invitrogen Antibodies
Targeting: FCER2 CD23, CD23A, CLEC4J, FCE2
Provider product page for 25-0238-41

Antibody data

Product number
25-0238-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD23 Monoclonal Antibody (EBVCS2), PE-Cyanine7, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The EBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21 and the alpha subunit of CD11b and CD11c bind to CD23. Applications Reported: This EBVCS2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This EBVCS2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
EBVCS2
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
FCER2 protein structure - 25-0238-41 shown in red.