HPA012800

PLD3 antibody from Atlas Antibodies

HU-K4

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Enhanced validation

Provider product page for HPA012800

Antibody Data

Product number

HPA012800

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA012800, RRID:AB_1855330

Product name

Anti-PLD3

Provider product page

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LDFPNASTGNPSTSQAWLGLLAGAHSSLDIASFYW
TLTNNDTHTQEPSAQQGEEVLRQLQTLAPKGVNVR
IAVSKPSGPQPQADLQALLQSGAQVRMVDMQKLTH
GVLHTKFWVVDQTHFYLGSAN


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

PLD3 is accumulated on neuritic plaques in Alzheimer’s disease brains
Satoh J, Kino Y, Yamamoto Y, Kawana N, Ishida T, Saito Y, Arima K
Alzheimer's Research & Therapy 2014 December;6(5-8)

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line SH-SY5Y.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and glial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows moderate to strong granular cytoplasmic positivity in neurons.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in lymphoid cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows moderate granular cytoplasmic positivity in islets of Langerhans.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows no granular cytoplasmic positivity in striated muscle fibers as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using HPA012800 antibody. Corresponding PLD3 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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