Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB100-93306 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-93306, RRID:AB_1236916
- Product name
- Rabbit Polyclonal eIF3X Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references CLUH granules coordinate translation of mitochondrial proteins with mTORC1 signaling and mitophagy.
CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins.
Pla-Martín D, Schatton D, Wiederstein JL, Marx MC, Khiati S, Krüger M, Rugarli EI
The EMBO journal 2020 May 4;39(9):e102731
The EMBO journal 2020 May 4;39(9):e102731
CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins.
Gao J, Schatton D, Martinelli P, Hansen H, Pla-Martin D, Barth E, Becker C, Altmueller J, Frommolt P, Sardiello M, Rugarli EI
The Journal of cell biology 2014 Oct 27;207(2):213-23
The Journal of cell biology 2014 Oct 27;207(2):213-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF3X Antibody [NB100-93306] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibody: Affinity purified rabbit anti-eIF3X antibody NB100-93306 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eIF3X was also immunoprecipitated by rabbit anti-eIF3X antibody NB100-93305, which recognizes an upstream epitope. For blotting immunoprecipitated eIF3X, the ReliaBLOT (R) Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).