Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- R34203 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- RCAN1 Antibody / Calcipressin 1
- Antibody type
- Polyclonal
- Description
- This highly specific RCAN1 antibody is suitable for use in Western blot/Immunofluorescence/Immunocytochemistry/Flow cytometry/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the RCAN1 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse brain and KO mouse brain lysates with RCAN1 antibody at 1ug/ml
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse brain and KO mouse brain lysates with RCAN1 antibody at 1ug/ml
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human cerebellum tissue lysate in the absence (A) and presence (B) of immunizing peptide, with RCAN1 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human A431 cells with RCAN1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human U-2 OS cells with RCAN1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human A431 cells with RCAN1 antibody (blue) at 10ug/ml and naive goat Ig (black).