Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-87599 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CEACAM4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FTIEALPSSA AEGKDVLLLA CNISETIQAY YWHKGKTAEG SPLIAGYITD IQANIPGAAY SGRETVYPNG SLLFQNITLE DAGSYTLRTI NASYDSDQAT GQLHVHQNNV PGLPVGAVAG; Positive Samples: U-87MG, A-549; Cellular Location: Membrane, Single-pass type I membrane protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.69 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of CEACAM4 in extracts of various cell lines. Samples were incubated with CEACAM4 Polyclonal antibody (Product # PA5-87599) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s.