711460

antibody from Invitrogen Antibodies

Targeting: DPPA3 Pgc7, Stella

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

711460

Provider

Invitrogen Antibodies

Product name

Stella Recombinant Polyclonal Antibody (17HCLC)

Antibody type

Polyclonal

Antigen

Other

Description

This antibody is predicted to react with Monkey, Rat and Horse.

Antibody clone number

17HCLC

Concentration

0.5 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, NCCIT cells were fixed and permeabilized for detection of endogenous DPPa3 using DPPa3 Recombinant Rabbit Polyclonal Antibody (Product # 711460, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of DPPa3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of DPPa3. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.