47-9818-42

antibody from Invitrogen Antibodies
Targeting: CLEC4C BDCA2, CD303, CLECSF11, CLECSF7, DLEC, HECL
Provider product page for 47-9818-42

Antibody data

Product number
47-9818-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD303a Monoclonal Antibody (201A), APC-eFluor™ 780, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This 201A monoclonal antibody reacts with human CD303a, which is also known as CLEC4C or Blood Dendritic Cell Antigen 2 (BDCA-2). This type II transmembrane protein is expressed almost exclusively on plasmacytoid dendritic cells, which secrete type I interferon during antiviral and antitumor responses. Studies have demonstrated that CD303a binds asialo-galactosyl-oligosaccharides on invading pathogens, resulting in the activation of signaling pathways that lead to CD303a internalization and downregulation of interferon production. Applications Reported: This 201A antibody has been reported for use in flow cytometric analysis. Applications Tested: This 201A antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
201A
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4°C, store in dark, DO NOT FREEZE!
CLEC4C protein structure - 47-9818-42 shown in red.