NBP1-22444

antibody from Novus Biologicals

Targeting: CACNA1H Cav3.2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-22444

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-22444, RRID:AB_1659986

Product name

Mouse Monoclonal Cav3.2 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects approx 260kDa. No cross-reactivity against Cav1.3.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Age attenuates the T-type Ca(V) 3.2-RyR axis in vascular smooth muscle.

Fan G, Kaßmann M, Cui Y, Matthaeus C, Kunz S, Zhong C, Zhu S, Xie Y, Tsvetkov D, Daumke O, Huang Y, Gollasch M
Aging cell 2020 Apr;19(4):e13134

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Brain-derived neurotrophic factor stimulation of T-type Ca²⁺ channels in sensory neurons contributes to increased peripheral pain sensitivity.

Wang H, Wei Y, Pu Y, Jiang D, Jiang X, Zhang Y, Tao J
Science signaling 2019 Sep 24;12(600)

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Cav3.2 calcium channel interactions with the epithelial sodium channel ENaC.

Garcia-Caballero A, Gandini MA, Huang S, Chen L, Souza IA, Dang YL, Stutts MJ, Zamponi GW
Molecular brain 2019 Feb 8;12(1):12

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Caveolae Link Ca_V3.2 Channels to BK_Ca-Mediated Feedback in Vascular Smooth Muscle.

Hashad AM, Harraz OF, Brett SE, Romero M, Kassmann M, Puglisi JL, Wilson SM, Gollasch M, Welsh DG
Arteriosclerosis, thrombosis, and vascular biology 2018 Oct;38(10):2371-2381

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T-type calcium channels functionally interact with spectrin (α/β) and ankyrin B.

Garcia-Caballero A, Zhang FX, Hodgkinson V, Huang J, Chen L, Souza IA, Cain S, Kass J, Alles S, Snutch TP, Zamponi GW
Molecular brain 2018 May 2;11(1):24

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TRPV1 Nociceptor Activity Initiates USP5/T-type Channel-Mediated Plasticity.

Stemkowski P, García-Caballero A, Gadotti VM, M'Dahoma S, Huang S, Black SAG, Chen L, Souza IA, Zhang Z, Zamponi GW
Cell reports 2016 Dec 13;17(11):2901-2912

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The deubiquitinating enzyme USP5 modulates neuropathic and inflammatory pain by enhancing Cav3.2 channel activity.

García-Caballero A, Gadotti VM, Stemkowski P, Weiss N, Souza IA, Hodgkinson V, Bladen C, Chen L, Hamid J, Pizzoccaro A, Deage M, François A, Bourinet E, Zamponi GW
Neuron 2014 Sep 3;83(5):1144-58

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cav3.2 Antibody (S55-10) [NBP1-22444] - Western Blot using rat brain membranes and NBP1-22444 to target Cav3.2 channels visible at about 260kD.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cav3.2 Antibody (S55-10) [NBP1-22444] - Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Cav3.2 Antibody (S55-10) [NBP1-22444] - Tissue: frozen brain section. Species: human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.