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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- PAB11442 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11442, RRID:AB_1713994
- Product name
- SIGLEC8 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of SIGLEC8.
- Antigen sequence
C-SYKSQLNYKTKQ- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement.
Ligation of Siglec-8: a selective mechanism for induction of human eosinophil apoptosis.
Yokoi H, Choi OH, Hubbard W, Lee HS, Canning BJ, Lee HH, Ryu SD, von Gunten S, Bickel CA, Hudson SA, Macglashan DW Jr, Bochner BS
The Journal of allergy and clinical immunology 2008 Feb;121(2):499-505.e1
The Journal of allergy and clinical immunology 2008 Feb;121(2):499-505.e1
Ligation of Siglec-8: a selective mechanism for induction of human eosinophil apoptosis.
Nutku E, Aizawa H, Hudson SA, Bochner BS
Blood 2003 Jun 15;101(12):5014-20
Blood 2003 Jun 15;101(12):5014-20
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- SIGLEC8 polyclonal antibody (Cat # PAB11442) (0.3 ug/mL) staining of MOLT 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
