347105

antibody from BioLegend

Targeting: SIGLEC8 MGC59785, SAF2, SIGLEC-8, SIGLEC8L

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

347105

Provider

BioLegend

Proper citation

BioLegend Cat#347105, RRID:AB_2561401

Product name

APC anti-human Siglec-8

Antibody type

Monoclonal

Antigen

Recombinant Siglec-8 fused to human IgG Fc

Description

Siglec-8 is a lectin specific for 6'-sulfo-sLex and a member of the Ig-superfamily. It is expressed almost exclusively in eosinophils; however, basophils and mast cells can express it to a lower degree. Siglec-8 is a 54 kD transmembranal protein; the extracellular domain has one V-set Ig-like domain and two C2-set domains. The cytoplasmic domain has two immunoreceptor tyrosine-based inhibitor motifs (ITIM) that recruit SH2-family phosphatases after tyrosine phosphorylation. There are reports that siglec-8 inhibits the release of histamine and prostaglandin D2 mediated by the IgEFcR. This molelcule is also involved in the induction of apoptosis.

Reactivity

Human

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Vial size

25 tests

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood leukocytes were stained with CCR3 PE and Siglec-8 (clone 7C9) APC (top) or mouse IgG1, κ isotype control (bottom). Data shown was gated on granulocyte cell population.

Conjugate

Red dye