Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1173 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat ICAM-5 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse ICAM-5 in direct ELISAs. Detects mouse and rat ICAM-5 in Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) ICAM-5 and recombinant mouse (rm) ICAM-2 is observed, 5% cross-reactivity with recombinant rat ICAM-1, rhICAM-1, and rhICAM-3 is observed and less than 1% cross-reactivity with rmICAM-1 and rmVCAM-1 is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q60625- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references MMPs and soluble ICAM-5 increase neuronal excitability within in vitro networks of hippocampal neurons.
Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
Matrix metalloproteinase-dependent shedding of intercellular adhesion molecule-5 occurs with long-term potentiation.
Niedringhaus M, Chen X, Dzakpasu R, Conant K
PloS one 2012;7(8):e42631
PloS one 2012;7(8):e42631
Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST
Journal of neurochemistry 2011 Aug;118(4):521-32
Journal of neurochemistry 2011 Aug;118(4):521-32
Matrix metalloproteinase-dependent shedding of intercellular adhesion molecule-5 occurs with long-term potentiation.
Conant K, Wang Y, Szklarczyk A, Dudak A, Mattson MP, Lim ST
Neuroscience 2010 Mar 17;166(2):508-21
Neuroscience 2010 Mar 17;166(2):508-21
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat ICAM-5 by Western Blot. Western blot shows lysates of rat olfactory bulb tissue, mouse olfactory bulb tissue, mouse brain tissue, and human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse ICAM-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1173) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ICAM-5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
