14-1579-80

antibody from Invitrogen Antibodies

Targeting: BST1 CD157

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

14-1579-80

Provider

Invitrogen Antibodies

Product name

Anti-CD157 Monoclonal Antibody (eBioSY11B5 (SY11B5)), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The eBioSY11B5 monoclonal antibody recognizes human CD157 (Mo5, BST-1). CD157 is a 42-45 kDa, GPI-anchored protein with structural and functional similarities with CD38. CD157 was initially cloned because of its expression on monocytes and macrophages, and was subsequently discovered to be the same protein named BST-1, discovered for its expression on bone marrow stromal cells and its ability to stimulate the proliferation of a mouse pre-B cell line. CD157 is a pleiotropic ectoenzyme and is thought to act independently as an enzyme and receptor. Similar to CD38, CD157 is involved in the metabolism of NAD+ and this activity may be involved in regulating intracellular Ca2+ levels. As a receptor, upon binding of its putative ligand, CD157 is thought to initiate a signal transduction cascade resulting in the phosphorylation of cytoplasmic proteins including focal adhesion kinase (FAK). The mechanism and functional significance of CD157-initiated signal transduction remain to be fully characterized. Applications Reported: This eBioSY11B5 (SY11B5) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) (non-reduced only), and immunohistology staining of frozen tissue sections. Applications Tested: This eBioSY11B5 (SY11B5) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

eBioSY11B5 (SY11B5)

Vial size

25 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

Enhancement of anti-leukemia activity of NK cells in vitro and in vivo by inhibition of leukemia cell-induced NK cell damage.

Arriga R, Caratelli S, Coppola A, Spagnoli GC, Venditti A, Amadori S, Lanzilli G, Lauro D, Palomba P, Sconocchia T, Del Principe MI, Maurillo L, Buccisano F, Capuani B, Ferrone S, Sconocchia G
Oncotarget 2016 Jan 12;7(2):2070-9

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Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells.

Aomatsu E, Takahashi N, Sawada S, Okubo N, Hasegawa T, Taira M, Miura H, Ishisaki A, Chosa N
Scientific reports 2014 Jan 13;4:3652

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CD157, the Janus of CD38 but with a unique personality.

Ortolan E, Vacca P, Capobianco A, Armando E, Crivellin F, Horenstein A, Malavasi F
Cell biochemistry and function 2002 Dec;20(4):309-22

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Genomic structure of human BST-1.

Muraoka O, Tanaka H, Itoh M, Ishihara K, Hirano T
Immunology letters 1996 Dec 1;54(1):1-4

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BST-1, a surface molecule of bone marrow stromal cell lines that facilitates pre-B-cell growth.

Kaisho T, Ishikawa J, Oritani K, Inazawa J, Tomizawa H, Muraoka O, Ochi T, Hirano T
Proceedings of the National Academy of Sciences of the United States of America 1994 Jun 7;91(12):5325-9

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Structural and biosynthetic features of the Mo5 human myeloid differentiation antigen.

Goldstein SC, Todd RF 3rd
Tissue antigens 1993 Apr;41(4):214-8

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (left) or 0.25 µg of Anti-Human CD157 Purified (right) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4010-82).