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- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- AF4736 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD157 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human CD157 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse CD157 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q10588- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Human canonical CD157/Bst1 is an alternatively spliced isoform masking a previously unidentified primate-specific exon included in a novel transcript.
Binding of CD157 protein to fibronectin regulates cell adhesion and spreading.
Ferrero E, Lo Buono N, Morone S, Parrotta R, Mancini C, Brusco A, Giacomino A, Augeri S, Rosal-Vela A, García-Rodríguez S, Zubiaur M, Sancho J, Fiorio Pla A, Funaro A
Scientific reports 2017 Nov 21;7(1):15923
Scientific reports 2017 Nov 21;7(1):15923
Binding of CD157 protein to fibronectin regulates cell adhesion and spreading.
Morone S, Augeri S, Cuccioloni M, Mozzicafreddo M, Angeletti M, Lo Buono N, Giacomino A, Ortolan E, Funaro A
The Journal of biological chemistry 2014 May 30;289(22):15588-601
The Journal of biological chemistry 2014 May 30;289(22):15588-601
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human CD157 by Western Blot. Western blot shows lysates of human kidney (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD157 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4736) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD157 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
