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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-51420 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DHRS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NKAFWETPAS MWDDINNVGL RGHYFCSVYG ARLMVPAGQG LIVVISSPGS LQYMFNVPYG VGKAACDKLA ADCAHELRRH GVSCVSLWPG IVQTELLKEH MAKEEVLQDP VLKQFKSAFS SAETTELSGK CVVALATDPN ILSLSGKVLP SCDLARRYGL RDVDGRPVQD YLSLSSVLSH VSGLGWLASY LPSFLRVPKW Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 83%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DHRS1 in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human cerebral cortex tissue. Samples were probed using a DHRS1 Polyclonal Antibody (Product # PA5-51420).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DHRS1 in Lane 1: Marker (kDa) 207, 110, 79, 49, 32, 25, 17; Lane 2: Human cell line RT-4; Lane 3: Human cell line EFO-21; Lane 4: Human cell line U-138MG (DSMZ); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a DHRS1 Polyclonal Antibody (Product # PA5-51420).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DHRS1 in human cell line U-2 OS shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a DHRS1 Polyclonal Antibody (Product # PA5-51420).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DHRS1 in human cell line A-431 using a DHRS1 Polyclonal Antibody (Product # PA5-51420) shows localization to nucleoplasm, cytosol and centrosome.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DHRS1 in human duodenum tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using a DHRS1 Polyclonal Antibody (Product # PA5-51420).
