Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF2859 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Siglec-6/CD327 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Siglec-6/CD327 in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Siglec-3, rhSiglec-5 and rhSiglec-9 is observed, approximately 5% cross-reactivity with rhSiglec-10 is observed, and less than 1% cross-reactivity with rhSiglec-2, rhSiglec-7, and rhSiglec-11 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_942142- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Glycodelin-A protein interacts with Siglec-6 protein to suppress trophoblast invasiveness by down-regulating extracellular signal-regulated kinase (ERK)/c-Jun signaling pathway.
Lam KK, Chiu PC, Lee CL, Pang RT, Leung CO, Koistinen H, Seppala M, Ho PC, Yeung WS
The Journal of biological chemistry 2011 Oct 28;286(43):37118-27
The Journal of biological chemistry 2011 Oct 28;286(43):37118-27
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human Siglec-6/CD327 ELISA Standard Curve. Recombinant Human Siglec-6/CD327 protein was serially diluted 2-fold and captured by Mouse Anti-Human Siglec-6/CD327 Monoclonal Antibody (Catalog # MAB28591) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Siglec-6/CD327 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2859) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
