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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB5806 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Siglec-E Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Siglec-E in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Siglec-3/CD33 or rmSiglec-F is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Siglec-7 restores β-cell function and survival and reduces inflammation in pancreatic islets from patients with diabetes.
Siglec-E Negatively Regulates the Activation of TLR4 by Controlling Its Endocytosis.
Dharmadhikari G, Stolz K, Hauke M, Morgan NG, Varki A, de Koning E, Kelm S, Maedler K
Scientific reports 2017 Apr 5;7:45319
Scientific reports 2017 Apr 5;7:45319
Siglec-E Negatively Regulates the Activation of TLR4 by Controlling Its Endocytosis.
Wu Y, Ren D, Chen GY
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):3336-3347
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):3336-3347
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse Siglec-E in Mouse Splenocytes. Mouse Splenocytes were stained with Anti-Mouse Integrin alpha X/CD11c Phycoerythrin-conjugated Antibody and either (A) Rat IgG2B Isotype Control (Catalog # MAB0061) or (B) Rat Anti-Mouse Siglec-E Monoclonal Antibody (Catalog # MAB5806) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
