Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- BAF1072 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Siglec-5/Siglec-14 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Siglec-5/Siglec-14 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 is observed and less than 5% cross-reactivity with rhSiglec-2 and rhSiglec-3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity.
Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.
Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5.
Pepin M, Mezouar S, Pegon J, Muczynski V, Adam F, Bianchini EP, Bazaa A, Proulle V, Rupin A, Paysant J, Panicot-Dubois L, Christophe OD, Dubois C, Lenting PJ, Denis CV
Scientific reports 2016 Nov 28;6:37953
Scientific reports 2016 Nov 28;6:37953
Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.
Zou Z, Chastain A, Moir S, Ford J, Trandem K, Martinelli E, Cicala C, Crocker P, Arthos J, Sun PD
PloS one 2011;6(9):e24559
PloS one 2011;6(9):e24559
Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5.
Carlin AF, Chang YC, Areschoug T, Lindahl G, Hurtado-Ziola N, King CC, Varki A, Nizet V
The Journal of experimental medicine 2009 Aug 3;206(8):1691-9
The Journal of experimental medicine 2009 Aug 3;206(8):1691-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Siglec-5 and Siglec-14 in Human Monocytes and Granulocytes by Flow Cytometry. Human whole blood monocytes and granulocytes were stained with Human Siglec-5/14 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1072, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).