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Western blot
Immunohistochemistry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-47058 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIGLEC5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 is observed and less than 1% cross-reactivity with rhSiglec-2 and rhSiglec-3 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Tumor stem cells fuse with monocytes to form highly invasive tumor-hybrid cells.
Aguirre LA, Montalbán-Hernández K, Avendaño-Ortiz J, Marín E, Lozano R, Toledano V, Sánchez-Maroto L, Terrón V, Valentín J, Pulido E, Casalvilla JC, Rubio C, Diekhorst L, Laso-García F, Del Fresno C, Collazo-Lorduy A, Jiménez-Munarriz B, Gómez-Campelo P, Llanos-González E, Fernández-Velasco M, Rodríguez-Antolín C, Pérez de Diego R, Cantero-Cid R, Hernádez-Jimenez E, Álvarez E, Rosas R, Dies López-Ayllón B, de Castro J, Wculek SK, Cubillos-Zapata C, Ibáñez de Cáceres I, Díaz-Agero P, Gutiérrez Fernández M, Paz de Miguel M, Sancho D, Schulte L, Perona R, Belda-Iniesta C, Boscá L, López-Collazo E
Oncoimmunology 2020 Jun 16;9(1):1773204
Oncoimmunology 2020 Jun 16;9(1):1773204
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SIGLEC5 in immersion fixed paraffin-embedded sections of human tonsil tissue. Samples were incubated with SIGLEC5 polyclonal antibody (Product # PA5-47058) using a dilution of 5 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal centers.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of SIGLEC5 in Human whole blood monocytes. Samples were incubated in SIGLEC5 polyclonal antibody (Product # PA5-47058) or control antibody followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of SIGLEC5 in human red blood cells. Samples were incubated in SIGLEC5 polyclonal antibody (Product # PA5-47058). Recombinant Human Siglec-5 Fc Chimera, immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Human Siglec-5/14 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.5-2.0 µg/mL.
