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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [5]
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- Product number
- NBP1-91149 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-91149, RRID:AB_11037914
- Product name
- Rabbit Polyclonal Siglec-5/CD170 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Siglec-5/CD170 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Siglec-5/CD170 Antibody [NBP1-91149] - Analysis in control (vector only transfected HEK293T lysate) and sIGLEC5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Siglec-5/CD170 Antibody [NBP1-91149] - Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subsets of bone marrow poietic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Siglec-5/CD170 Antibody [NBP1-91149] - Staining of human bone marrow shows strong cytoplasm granular positivity in hematopoietic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Siglec-5/CD170 Antibody [NBP1-91149] - Staining of human smooth muscle shows weak cytoplasmic positivity in smooth muscle cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Siglec-5/CD170 Antibody [NBP1-91149] - Staining of human tonsil shows moderate cytoplasm granular positivity in non-germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Siglec-5/CD170 Antibody [NBP1-91149] - Staining of human stomach shows no cytoplasmic positivity in glandular cells as expected.
