Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF738 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Angiopoietin-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Angiopoietin-3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Angiopoietin-2 or rhAngoipoietin-3 was observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Angiopoietin-3 in Rat Kidney. Angiopoietin-3 was detected in perfusion fixed frozen sections of rat kidney using 5 µg/mL Goat Anti-Mouse Angiopoietin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF738) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.