Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS60080 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- GADD45B polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to residues in Human GADD45B.
- Description
- Cellcycle progression issubject to arrest at G1 and G2 checkpointsin response to DNA damage, presumablyto allow time for DNA repair prior to entryinto S andMphase, respectively. The p53 tumorsuppressor isrequired for one such G1 checkpoint and functionsto upregulate expression of GADD 45 and p21. GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair. GADD 45 stimulates DNA excision repair in vitro and inhibits entry ofcellsinto S phase. Thus, it has been suggested that GADD 45 mayserve as a link between the p53-dependentcellcycle checkpoint and DNA repair. GADD 45-like proteins, GADD 45ÉÇ and GADD 45ÎÛ, have been shown to be induced by environmentalstresses. GADD 45ÉÇ and GADD 45ÎÛ are thought to induce p38/JNK activation via MEKK4 activation.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of GADD45B pAb at 1:500 dilutionLane1:MCF-7 whole cell lysate Lane2:NIH-3T3 whole cell lysateLane3:H9C2 whole cell lysate