PA5-43160

antibody from Invitrogen Antibodies

Targeting: GADD45B DKFZP566B133, GADD45BETA, MYD118

Western blot

Immunohistochemistry

Antibody data

Submit

Validation data

Product number: PA5-43160 - Provider product page
Provider: Invitrogen Antibodies
Product name: GADD45B Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Peptide sequence: FCCDNDINIV RVSGMQRLAQ LLGEPAETQG TTEARDLHCL LVTNPHTDAW Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

GADD45B protein structure - PA5-43160 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 The expression of growth arrest and DNA damage-inducible beta (GADD45B) was increased in the kidneys of db/db mice and HG-induced HK-2 cells. (A) Heat map of the 1,194 differentially expressed genes (the logarithm of the fold change to base 2 > 2) and the right part is the enlarged view containing GADD45B. (B) The logarithm of the fold change to base 2 of GADD45B expression was determined by RNA sequencing analysis. (C) Real-time PCR (RT-PCR) analysis of GADD45B messenger RNA (mRNA) expression in kidney samples, N = 9. (D) Representative images of immunohistochemical staining of GADD45B in kidney sections. Original magnification = 400. Scale bar = 100 mum. (E,F) Western blot bands and quantitative analysis of GADD45B protein expression in kidney samples, N = 6. (G,H) RT-PCR analysis of GADD45B mRNA expression in HK-2 cells, N = 3. (I-L) Western blot bands and quantitative analysis of GADD45B protein expression in HK-2 cells, N = 3. The data are presented as the mean +- SD. * p < 0.05.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Effects of GADD45B knockdown on renal tubular epithelial-mesenchymal transition (EMT) and apoptosis in HK-2 cells under high glucose (HG) conditions. (A) Representative images of immunofluorescence staining of GADD45B in HK-2 cells. Original magnification = 200. Scale bar = 200 mum. (B) Western blot bands showing E-cadherin, Vimentin, and alpha-smooth muscle actin (alpha-SMA) protein expression in HK-2 cells. (C) Quantitative analysis of (B) , N = 3. (D) Western blot bands showing Bax, Bcl2, and cleaved Caspase 3 protein expression in HK-2 cells. (E) Quantitative analysis of (D) , N = 3. The data are presented as the mean +- SD. * p < 0.05.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Effects of GADD45B overexpression on renal tubular EMT and apoptosis in HK-2 cells. (A) Representative images of immunofluorescence staining of GADD45B in HK-2 cells. Original magnification = 200. Scale bar = 200 mum. (B) Western blot bands showing E-cadherin, Vimentin, and alpha-SMA protein expression in HK-2 cells. (C) Quantitative analysis of (B) , N = 4. (D) Western blot bands showing Bax, Bcl2, and cleaved Caspase 3 protein expression in HK-2 cells. (E) Quantitative analysis of (D), N = 4. The data are presented as the mean +- SD. * p < 0.05.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4. Changes of neuroprotective molecules after global ischemia. (A) Wnt family mRNA expression showing time-dependent alterations in ischemia hippocampal CA1 regions as determined by quantitative real time-polymerase chain reaction (qRT-PCR). (B) Growth arrest and DNA-damage-inducible protein 45 beta (Gadd45b) mRNA expression quantified by qRT-PCR. (C) Representative immunoblot images of Gadd45b in hippocampal CA1 with complementary quantifications normalized by tubulin (n=7 for sham, 4 for ischemia 24- and 48-hour animals). Values represent mean +-standard error of the mean (*P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 5. Knockdown of growth arrest and DNA-damage-inducible protein 45 beta (Gadd45b) accelerates neuronal cell death and mitochondrial dysfunction. (A) Validation of knockdown efficiency for Gadd45b shRNA in cortical neurons by immunoblotting. (B) Summary data of lactate dehydrogenase (LDH) activity assay for cortical neurons treated with 50uM H 2 O 2 (2 hours) in the presence of nontargeting (NT) or Gadd45b shRNA. LDH activity values are normalized to the value of cells exposed to 400muM H 2 O 2 (100%, FK; full kill) after subtracting the value of control cells (0%). (C) Representative immunoblot images showing Bcl-2. (D) Bax expression level in cortical neurons treated with 50uM H 2 O 2 (2 hours) in the presence of NT or Gadd45b shRNA. (E) Quantitative real timepolymerase chain reaction data showing brain-derived neurotrophic factor (BDNF) mRNA expression at indicated time periods after ischemia. (F) Representative immunoblot images Gadd45b in hippocampal CA1 and summary data. (n=7 for sham, 4 for ischemia 24- and 48-hour animals). Values represent mean+-standard error of themean. *P