TA328654

antibody from OriGene

Targeting: TRPV3 VRL3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA328654

Provider

OriGene

Product name

Rabbit Polyclonal Anti-TRPV3 (extracellular)

Antibody type

Polyclonal

Description

Rabbit Polyclonal Anti-TRPV3 (extracellular)

Host

Rabbit

Conjugate

Unconjugated

Epitope

TRPV3

Antibody clone number

NULL

Vial size

200 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of rat DRG (lanes 1 and 2) and rat brain (lanes 3 and 4) lysates: 1, 3. Anti-TRPV3 (extracellular) antibody, (1:200). 2, 4. Anti-TRPV3 (extracellular) antibody, preincubated with the control peptide antigen.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Expression of TRPV3 in rat DRG primary culture. Immunocytochemical staining of living rat dorsal root ganglion (DRG) primary culture using Anti-TRPV3 (extracellular) antibody, (1:50-1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (A). B. Nuclear staining with the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IHC staining of mouse spinal cord using Anti-TRPV3 (extracellular) antibody. A. TRPV3 (green) appears in neurons (vertical arrows) in the ventral horn of the mouse spinal cord. B. Motor neurons were stained with goat anti choline-acetyltransferase (red). In some motor neurons (A) TRPV3 was more intense (vertical arrow) whereas in others it was weak (horizontal arrows). C. Merged images of panels A and B. The inset in C magnifies one large motor neuron (vertical arrowhead).

Validation comment

IHC