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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-27597 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPV3 Monoclonal Antibody (N15/39)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- 1 µg/mL of MA5-27597 was sufficient for detection of TrpV3 in 10 µg of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody|Detects approximately 70kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- N15/39
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRPV3 in rat brain membrane lysate with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with TRPV3 monoclonal antibody (Product # MA5-27597) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TRPV3 in human HaCaT cells. Sample was fixed using 100% cold methanol (10 minutes at -20°C), incubated with TRPV3 monoclonal antibody (Product # MA5-27597) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRPV3 in paraffin-embedded mouse back skin. Samples were incubated with TRPV3 monoclonal antibody (Product # MA5-27597) using a dilution of 1:100 (1 hour at RT) and followed by FITC Goat Anti-Rabbit (green) at a dilution of 1:50 (1 hour at RT).
