Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B16351 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ SGTA / SGT Antibody (clone AT19E8) LS-B16351
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AT19E8
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SGTA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: K562 cell lysate Lane 3.: A549 cell lysate Lane 4.: MCF7 cell lysate Lane 5.: LNCaP cell lysate Lane 6.: 293T cell lysate