11019-2-AP

antibody from Invitrogen Antibodies

Targeting: SGTA SGT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11019-2-AP

Provider

Invitrogen Antibodies

Product name

SGTA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MDNKKRLAY AIIQFLHDQL RHGGLSSDAQ ESLEVAIQCL ETAFGVTVED SDLALPQTLP EIFEAAATGK EMPQDLRSPA RTPPSEEDSA EAERLKTEGN EQMKVENFEA AVHFYGKAIE LNPANAVYFC NRAAAYSKLG NYAGAVQDCE RAICIDPAYS KAYGRMGLAL SSLNKHVEAV AYYKKALELD PDNETYKSNL KIAELKLREA PSPTGGVGSF DIAGLLNNPG FMSMASNLMN NPQIQQLMSG MISGGNNPLG TPGTSPSQND LASLIQAGQQ FAQQMQQQNP ELIEQLRSQI RSRTPSASND DQQE (1-313 aa encoded by BC008885)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 11019-2-AP (SGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 11019-2-AP (SGTA antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SGTA (IP:11019-2-AP, 4ug; Detection:11019-2-AP 1:1000) with HeLa cells lysate 2480ug.