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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 60305-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SGTA Monoclonal Antibody (3B10B11)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MDNKKRLAY AIIQFLHDQL RHGGLSSDAQ ESLEVAIQCL ETAFGVTVED SDLALPQTLP EIFEAAATGK EMPQDLRSPA RTPPSEEDSA EAERLKTEGN EQMKVENFEA AVHFYGKAIE LNPANAVYFC NRAAAYSKLG NYAGAVQDCE RAICIDPAYS KAYGRMGLAL SSLNKHVEAV AYYKKALELD PDNETYKSNL KIAELKLREA PSPTGGVGSF DIAGLLNNPG FMSMASNLMN NPQIQQLMSG MISGGNNPLG TPGTSPSQND LASLIQAGQQ FAQQMQQQNP ELIEQLRSQI RSRTPSASND DQQE (1-313 aa encoded by BC008885)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3B10B11
- Vial size
- 150 µL
- Concentration
- 2.03 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 60305-1-IG ( SGTA Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 60305-1-IG (SGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 60305-1-IG ( SGTA Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 60305-1-IG ( SGTA Antibody) at dilution of 1:200 (under 40x lens).
