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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-30171 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SGTA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116, mouse liver. Predicted reactivity: Mouse (88%), Rat (87%), Dog (95%), Pig (95%), Chicken (88%), Rhesus Monkey (100%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.69 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SGTA Polyclonal Antibody (Product # PA5-30171). Sample (30 µg of whole cell lysate). Lane A: A549. 10% SDS PAGE. SGTA Polyclonal Antibody (Product # PA5-30171) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SGTA Polyclonal Antibody (Product # PA5-30171). Sample (50 µg of whole cell lysate). Lane A: mouse liver. 10% SDS PAGE. SGTA Polyclonal Antibody (Product # PA5-30171) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SGTA in methanol-fixed HeLa cells using a SGTA polyclonal antibody (Product # PA5-30171) at a 1:200 dilution.
