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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-21327 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCARA3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2.
- Concentration
- 0.78 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SCARA3 Polyclonal Antibody (Product # PA5-21327). Sample (30 µg of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. SCARA3 Polyclonal Antibody (Product # PA5-21327) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SCARA3 Polyclonal Antibody detects SCARA3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20ºC 100% MeOH for 5 min. Green: SCARA3 protein stained by SCARA3 Polyclonal Antibody (Product # PA5-21327) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using SCARA3 (Product # PA5-21327) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
