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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PAB2050 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2050, RRID:AB_1574254
- Product name
- DCLK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of DCLK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of a novel putative gastrointestinal stem cell and adenoma stem cell marker, doublecortin and CaM kinase-like-1, following radiation injury and in adenomatous polyposis coli/multiple intestinal neoplasia mice.
May R, Riehl TE, Hunt C, Sureban SM, Anant S, Houchen CW
Stem cells (Dayton, Ohio) 2008 Mar;26(3):630-7
Stem cells (Dayton, Ohio) 2008 Mar;26(3):630-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The DCLK1 polyclonal antibody (Cat # PAB2050) is used in Western blot to detect DCLK1 in mouse heart tissue lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ZR-75-1 cell lysate (35 ug/lane) with DCLK1 polyclonal antibody (Cat # PAB2050).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Expression pattern of doublecortin and DCLK1 in normal mouse small intestine.A : Immunohistochemical staining of normal small intestine for DCLK1. Arrows indicate the cells positive for DCLK1 in the stem cell zone.B : Preincubation with blocking peptide completely abolished DCLK1 immunoreactivity.C : Immunohistochemical staining of normal small intestine for DCLK1. Brown indicates the cells positive for DCLK1 (indicated by the arrows).
- Validation comment
- Immunohistochemistry
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with DCLK1 polyclonal antibody (Cat # PAB2050), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
