HPA015655

antibody from Atlas Antibodies

Targeting: DCLK1 DCAMKL1, DCDC3A, DCLK, KIAA0369

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA015655

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA015655, RRID:AB_2669265

Product name

Anti-DCLK1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

TSSVNGTPGSQLSTPRSGKSPSPSPTSPGSLRKQR
SSQHGGSSTSLASTKVCSSMDENDGPGEEVSEEGF
QIPATITERYKVGRTIGDGNFAVVKECVERSTARE
YALKIIK


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human cerebral cortex and kidney tissues using Anti-DCLK1 antibody. Corresponding DCLK1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows low expression as expected.

Sample type

HUMAN