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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-24783 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Thimet Oligopeptidase Monoclonal Antibody (4D6)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D6
- Vial size
- 100 µL
- Concentration
- 1.54 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Thimet Oligopeptidase/THOP1 in HeLa cells using 30 µg of sample per lane. The membrane was probed with Thimet Oligopeptidase/THOP1 monoclonal antibody (Product # MA5-24783) using a dilution of Lane 1) 0.5 µg/mL and Lane 2) 2 µg/mL. Chemiluminescent detection was performed using a 1 minute exposure time.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Thimet Oligopeptidase in 30 µg HeLa whole cell lysate. Samples were incubated in Thimet Oligopeptidase monoclonal antibody (Product # MA5-24783). Lane 1: 0.5 µg/mL antibody; lane 2: 2 µg/mL antibody. 1 minute ECL exposure.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Thimet Oligopeptidase in mouse brain. Samples were incubated with Thimet Oligopeptidase monoclonal antibody (Product # MA5-24783) followed by using DAB with hematoxylin counterstain.
