NBP1-49311AF488

antibody from Novus Biologicals

Targeting: CLEC4E CLECSF9, mincle

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-49311AF488

Provider

Novus Biologicals

Product name

Mouse Monoclonal CLEC4E Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Rat

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C in the dark.

References

Submitted references

C-type lectin receptors Mcl and Mincle control development of multiple sclerosis-like neuroinflammation.

N'diaye M, Brauner S, Flytzani S, Kular L, Warnecke A, Adzemovic MZ, Piket E, Min JH, Edwards W, Mela F, Choi HY, Magg V, James T, Linden M, Reichardt HM, Daws MR, van Horssen J, Kockum I, Harris RA, Olsson T, Guerreiro-Cacais AO, Jagodic M
The Journal of clinical investigation 2020 Feb 3;130(2):838-852

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CLEC4E Antibody (16E3) [Alexa Fluor® 488] [NBP1-49311AF488] - An intracellular stain was performed on U-937 cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CLEC4E Antibody (16E3) [Alexa Fluor® 488] [NBP1-49311AF488] - An intracellular stain was performed on U87MG cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CLEC4E Antibody (16E3) [Alexa Fluor® 488] [NBP1-49311AF488] - Activated human blood mononuclear cells (PBMCs). Image from verified customer review.