Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM09361PU-N - Provider product page
- Provider
- OriGene
- Product name
- CLEC4E mouse monoclonal antibody, clone AT16E3, Purified
- Antibody type
- Monoclonal
- Description
- CLEC4E mouse monoclonal antibody, clone AT16E3, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CLEC4E
- Isotype
- IgG
- Antibody clone number
- AT16E3
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
Submitted references The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression.
Seifert L, Werba G, Tiwari S, Giao Ly NN, Alothman S, Alqunaibit D, Avanzi A, Barilla R, Daley D, Greco SH, Torres-Hernandez A, Pergamo M, Ochi A, Zambirinis CP, Pansari M, Rendon M, Tippens D, Hundeyin M, Mani VR, Hajdu C, Engle D, Miller G
Nature 2016 Apr 14;532(7598):245-9
Nature 2016 Apr 14;532(7598):245-9
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MINCLE (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of human HeLa cells stained with monoclonal anti-human MINCLE antibody (1/500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Paraffin embedded sections of colorectal cancer tissue were incubated with anti-Human MINCLE antibody (1/50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
- Validation comment
- IHC
