MAB8479

antibody from Abnova Corporation

Targeting: CLEC4E CLECSF9, mincle

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB8479

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB8479, RRID:AB_10721495

Product name

CLEC4E monoclonal antibody, clone AT16E3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant CLEC4E.

Isotype

IgG

Antibody clone number

AT16E3

Storage

Store at 4°C short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The Dectin-2 family of C-type lectins in immunity and homeostasis.

Graham LM, Brown GD
Cytokine 2009 Oct-Nov;48(1-2):148-55

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Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle.

Ishikawa E, Ishikawa T, Morita YS, Toyonaga K, Yamada H, Takeuchi O, Kinoshita T, Akira S, Yoshikai Y, Yamasaki S
The Journal of experimental medicine 2009 Dec 21;206(13):2879-88

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Mincle is a long sought receptor for mycobacterial cord factor.

Matsunaga I, Moody DB
The Journal of experimental medicine 2009 Dec 21;206(13):2865-8

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with CLEC4E monoclonal antibody, clone AT16E3 (Cat # MAB8479) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of human HeLa cells stained with monoclonal CLEC4E monoclonal antibody, clone AT16E3 (Cat # MAB8479) (1 : 500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with monoclonal CLEC4E monoclonal antibody, clone AT16E3 (Cat # MAB8479) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)