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LearnPA5-34434
antibody from Invitrogen Antibodies
Targeting: PHLPP1
KIAA0606, PHLPP, PLEKHE1, PPM3A, SCOP
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [4]
- Other assay [2]
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- Product number
- PA5-34434 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHLPP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is SW480 cell lysate. PA5-34434 can be used with blocking peptide PEP-1476.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references USP46 Inhibits Cell Proliferation in Lung Cancer through PHLPP1/AKT Pathway.
Wang W, Chen M, Xu H, Lv D, Zhou S, Yang H
BioMed research international 2020;2020:2509529
BioMed research international 2020;2020:2509529
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of SW480 cells using a PHLPP1 polyclonal antibody (Product # PA5-34434) at a 25 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SW480 cells using a PHLPP1 polyclonal antibody (Product # PA5-34434) at a 5 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of PHLPP1 in SW480 cells with PHLPP1 Polyclonal Antibody (Product # PA5-34434) at 2.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of PHLPP1 in SW480 cells with PHLPP1 Polyclonal Antibody (Product # PA5-34434) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 USP46 and PHLPP1 were downregulated in human lung cancer tissues. (a) mRNA level of USP46 in human lung cancer and adjacent-matched tissues was measured by qRT-PCR. USP46 mRNA levels were normalized to the beta-actin mRNA levels ( n = 30). ** means p < 0.01. (b) Expressions of USP46 and PHLPP1 in 8 pairs of human lung cancer and adjacent-matched tissues were measured by western blotting (left), and protein levels were quantified according to the gray value after normalization to beta -actin ( n = 8). *** means p < 0.001.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 USP46 promoted PHLPP1 protein stability via deubiquitination in human lung cancer cells. (a, b) mRNA levels of PHLPP1 in A549 and H1299 cells upon USP46 overexpression (a) and USP46 knockdown (b) were measured by RT-PCR. The data were normalized to beta-actin. Mean +- standard deviation (SD) ( n = 3). (c) Interaction between USP46 and PHLPP1 was tested by co-IP followed by WB using indicated antibodies. (d) USP46-dependent ubiquitination in PHLPP1 was measured by comparing ubiquitination in PHLPP1 in USP46 overexpressing cells (oeUSP46) and control cells (oeNC).
