22789-1-AP

antibody from Proteintech Group

Targeting: PHLPP1 KIAA0606, PHLPP, PLEKHE1, PPM3A, SCOP

Provider product page for 22789-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [32]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]

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Product number: 22789-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#22789-1-AP, RRID:AB_2750897
Product name: PHLPP antibody
Antibody type: Polyclonal
Description: KD/KO validated PHLPP antibody (Cat. #22789-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PHLPP1 protein structure - 22789-1-AP shown in red.

Submitted references A multiprotein signaling complex sustains AKT and mTOR/S6K activity necessary for the survival of cancer cells undergoing stress.

Teran Pumar OY, Zanotelli MR, Lin MJ, Schmitt RR, Green KS, Rojas KS, Hwang IY, Cerione RA, Wilson KF
bioRxiv : the preprint server for biology 2024 Oct 1;

Corynoxine triggers cell death via activating PP2A and regulating AKT-mTOR/GSK3β axes in NSCLC.

Hou G, Hu W, Sang Y, Gan X, Xu H, Hu Q, Cao X
Biochemical pharmacology 2024 Apr;222:116110

Blockage of PHLPP1 protects against myocardial ischemia/reperfusion injury in diabetic mice via activation of STAT3 signaling.

Gao S, Qiu Y, Meng Y, Jia Y, Lang X, Zhao H, Sun H, Zhang J, Ding L
Journal of bioenergetics and biomembranes 2023 Oct;55(5):325-339

NAP1L5 facilitates pancreatic ductal adenocarcinoma progression via TRIM29-mediated ubiquitination of PHLPP1.

Xiao B, Ge Y, Zhao R, Zhang Y, Guo Y, Zhang S, Li B, Qiu P, Chao Z, Zuo S
Biochemical pharmacology 2023 Nov;217:115811

FKBP51 plays an essential role in Akt ubiquitination that requires Hsp90 and PHLPP.

Tufano M, Marrone L, D'Ambrosio C, Di Giacomo V, Urzini S, Xiao Y, Matuozzo M, Scaloni A, Romano MF, Romano S
Cell death & disease 2023 Feb 13;14(2):116

Hyperglycemia-Induced Overexpression of PH Domain Leucine-Rich Repeat Protein Phosphatase 1 (PHLPP1) Compromises the Cardioprotective Effect of Ischemic Postconditioning Via Modulation of the Akt/Mst1 Pathway Signaling.

Qiu Y, Meng Y, Jia Y, Lang X, Zhao H, Ding L, Wang T, Sun H, Gao S
Cardiovascular drugs and therapy 2023 Dec;37(6):1087-1101

Single-residue mutation in protein kinase C toggles between cancer and neurodegeneration.

Jones AC, Kornev AP, Weng JH, Manning G, Taylor SS, Newton AC
The Biochemical journal 2023 Aug 30;480(16):1299-1316

Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload.

Qin X, Fu Y, Fan J, Liu B, Liu P, Zhang Y, Jiang T, Zheng Q
Journal of pineal research 2023 Apr;74(3):e12851

Low-Intensity Pulsed Ultrasound Attenuates Postoperative Neurocognitive Impairment and Salvages Hippocampal Synaptogenesis in Aged Mice.

Wang Q, Liu T, Chang H, Li Z, Chen L, Mi X, Xing H, Wang X, Hong J, Liu K, Li Y, Han D, Li Y, Yang N, Li X, Li Y, Guo X
Brain sciences 2023 Apr 13;13(4)

Circular RNA circRASSF5 Functions as an Anti-Oncogenic Factor in Hepatocellular Carcinoma by Acting as a Competitive Endogenous RNA Through Sponging miR-331-3p.

Zhou Z, Cui X, Gao P, Zhang X, Zhu C, Sun B
Journal of hepatocellular carcinoma 2022;9:1041-1056

PHLPP1 regulates CFTR activity and lumen expansion through AMPK.

Lobert VH, Skardal ML, Malerød L, Simensen JE, Algra HA, Andersen AN, Fleischer T, Enserink HA, Liestøl K, Heath JK, Rusten TE, Stenmark HA
Development (Cambridge, England) 2022 Oct 15;149(20)

The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness.

Thomas GE, Egan G, García-Prat L, Botham A, Voisin V, Patel PS, Hoff FW, Chin J, Nachmias B, Kaufmann KB, Khan DH, Hurren R, Wang X, Gronda M, MacLean N, O'Brien C, Singh RP, Jones CL, Harding SM, Raught B, Arruda A, Minden MD, Bader GD, Hakem R, Kornblau S, Dick JE, Schimmer AD
Nature cell biology 2022 Jun;24(6):872-884

Reduced PHLPP Expression Leads to EGFR-TKI Resistance in Lung Cancer by Activating PI3K-AKT and MAPK-ERK Dual Signaling.

Wang W, Xia X, Chen K, Chen M, Meng Y, Lv D, Yang H
Frontiers in oncology 2021;11:665045

Chondrocytic and pharmacokinetic properties of Phlpp inhibitors.

Taylor EL, Weaver SR, Zars EL, Turner CA, Buhrow SA, Reid JM, Bradley EW, Westendorf JJ
Osteoarthritis and cartilage open 2021 Sep;3(3):100190

Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.

Suryawan A, El-Kadi SW, Nguyen HV, Fiorotto ML, Davis TA
The Journal of nutrition 2021 Sep 4;151(9):2636-2645

Basolateral protein Scribble binds phosphatase PP1 to establish a signaling network maintaining apicobasal polarity.

Troyanovsky RB, Indra I, Kato R, Mitchell BJ, Troyanovsky SM
The Journal of biological chemistry 2021 Nov;297(5):101289

CUL4B negatively regulates Toll-like receptor-triggered proinflammatory responses by repressing Pten transcription.

Song Y, Li P, Qin L, Xu Z, Jiang B, Ma C, Shao C, Gong Y
Cellular & molecular immunology 2021 Feb;18(2):339-349

The PHLPP1 N-Terminal Extension Is a Mitotic Cdk1 Substrate and Controls an Interactome Switch.

Kawashima AT, Wong C, Lordén G, King CC, Lara-Gonzalez P, Desai A, Gingras AC, Newton AC
Molecular and cellular biology 2021 Feb 23;41(3):e0033320

CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.

Jiang T, Wang H, Liu L, Song H, Zhang Y, Wang J, Liu L, Xu T, Fan R, Xu Y, Wang S, Shi L, Zheng L, Wang R, Song J
Molecular cancer 2021 Dec 18;20(1):167

Inhibition of PHLPP1/2 phosphatases rescues pancreatic β-cells in diabetes.

Lupse B, Annamalai K, Ibrahim H, Kaur S, Geravandi S, Sarma B, Pal A, Awal S, Joshi A, Rafizadeh S, Madduri MK, Khazaei M, Liu H, Yuan T, He W, Gorrepati KDD, Azizi Z, Qi Q, Ye K, Oberholzer J, Maedler K, Ardestani A
Cell reports 2021 Aug 3;36(5):109490

Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

He X, Li M, Yu H, Liu G, Wang N, Yin C, Tu Q, Narla G, Tao Y, Cheng S, Yin H
PLoS biology 2020 Dec;18(12):e3000803

Cigarette smoke extract induces airway epithelial cell death via repressing PRMT6/AKT signaling.

Li T, Fanning KV, Nyunoya T, Chen Y, Zou C
Aging 2020 Dec 1;12(23):24301-24317

Inhibition of PHLPP1 ameliorates cardiac dysfunction via activation of the PI3K/Akt/mTOR signalling pathway in diabetic cardiomyopathy.

Zhang M, Wang X, Liu M, Liu D, Pan J, Tian J, Jin T, Xu Y, An F
Journal of cellular and molecular medicine 2020 Apr;24(8):4612-4623

USP1 deubiquitinates Akt to inhibit PI3K-Akt-FoxO signaling in muscle during prolonged starvation.

Goldbraikh D, Neufeld D, Eid-Mutlak Y, Lasry I, Gilda JE, Parnis A, Cohen S
EMBO reports 2020 Apr 3;21(4):e48791

Phlpp1 is associated with human intervertebral disc degeneration and its deficiency promotes healing after needle puncture injury in mice.

Zhang C, Smith MP, Zhou GK, Lai A, Hoy RC, Mroz V, Torre OM, Laudier DM, Bradley EW, Westendorf JJ, Iatridis JC, Illien-Jünger S
Cell death & disease 2019 Oct 3;10(10):754

PHLPP1 counter-regulates STAT1-mediated inflammatory signaling.

Cohen Katsenelson K, Stender JD, Kawashima AT, Lordén G, Uchiyama S, Nizet V, Glass CK, Newton AC
eLife 2019 Aug 13;8

Protein Kinase C Quality Control by Phosphatase PHLPP1 Unveils Loss-of-Function Mechanism in Cancer.

Baffi TR, Van AN, Zhao W, Mills GB, Newton AC
Molecular cell 2019 Apr 18;74(2):378-392.e5

PHLPP regulates hexokinase 2-dependent glucose metabolism in colon cancer cells.

Xiong X, Wen YA, Mitov MI, C Oaks M, Miyamoto S, Gao T
Cell death discovery 2017;3:16103

High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.

Xie Y, Lv D, Wang W, Ye M, Chen X, Yang H
Oncotarget 2017 Aug 29;8(35):59000-59007

Pleckstrin Homology (PH) Domain Leucine-rich Repeat Protein Phosphatase Controls Cell Polarity by Negatively Regulating the Activity of Atypical Protein Kinase C.

Xiong X, Li X, Wen YA, Gao T
The Journal of biological chemistry 2016 Nov 25;291(48):25167-25178

PHLPP negatively regulates cell motility through inhibition of Akt activity and integrin expression in pancreatic cancer cells.

Smith AJ, Wen YA, Stevens PD, Liu J, Wang C, Gao T
Oncotarget 2016 Feb 16;7(7):7801-15

Loss of PHLPP protects against colitis by inhibiting intestinal epithelial cell apoptosis.

Wen YA, Li X, Goretsky T, Weiss HL, Barrett TA, Gao T
Biochimica et biophysica acta 2015 Oct;1852(10 Pt A):2013-23

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse brain tissue were subjected to SDS PAGE followed by western blot with 22789-1-AP(PHLPP antibody) at dilution of 1:1000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using PHLPP antibody 22789-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-PHLPP (IP:22789-1-AP, 5ug; Detection:22789-1-AP 1:1000) with mouse brain tissue lysate 5000ug.
Sample type: tissue