Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- 22789-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#22789-1-AP, RRID:AB_2750897
- Product name
- PHLPP antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated PHLPP antibody (Cat. #22789-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Reduced PHLPP Expression Leads to EGFR-TKI Resistance in Lung Cancer by Activating PI3K-AKT and MAPK-ERK Dual Signaling.
Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.
Basolateral protein Scribble binds phosphatase PP1 to establish a signaling network maintaining apicobasal polarity.
The PHLPP1 N-Terminal Extension is a Mitotic Cdk1 Substrate and Controls an Interactome Switch.
CUL4B negatively regulates Toll-like receptor-triggered proinflammatory responses by repressing Pten transcription.
Inhibition of PHLPP1/2 phosphatases rescues pancreatic β-cells in diabetes.
Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.
Cigarette smoke extract induces airway epithelial cell death via repressing PRMT6/AKT signaling.
Inhibition of PHLPP1 ameliorates cardiac dysfunction via activation of the PI3K/Akt/mTOR signalling pathway in diabetic cardiomyopathy.
USP1 deubiquitinates Akt to inhibit PI3K-Akt-FoxO signaling in muscle during prolonged starvation.
Phlpp1 is associated with human intervertebral disc degeneration and its deficiency promotes healing after needle puncture injury in mice.
PHLPP1 counter-regulates STAT1-mediated inflammatory signaling.
Protein Kinase C Quality Control by Phosphatase PHLPP1 Unveils Loss-of-Function Mechanism in Cancer.
PHLPP regulates hexokinase 2-dependent glucose metabolism in colon cancer cells.
High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.
Pleckstrin Homology (PH) Domain Leucine-rich Repeat Protein Phosphatase Controls Cell Polarity by Negatively Regulating the Activity of Atypical Protein Kinase C.
PHLPP negatively regulates cell motility through inhibition of Akt activity and integrin expression in pancreatic cancer cells.
Loss of PHLPP protects against colitis by inhibiting intestinal epithelial cell apoptosis.
Wang W, Xia X, Chen K, Chen M, Meng Y, Lv D, Yang H
Frontiers in oncology 2021;11:665045
Frontiers in oncology 2021;11:665045
Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.
Suryawan A, El-Kadi SW, Nguyen HV, Fiorotto ML, Davis TA
The Journal of nutrition 2021 Sep 4;151(9):2636-2645
The Journal of nutrition 2021 Sep 4;151(9):2636-2645
Basolateral protein Scribble binds phosphatase PP1 to establish a signaling network maintaining apicobasal polarity.
Troyanovsky RB, Indra I, Kato R, Mitchell BJ, Troyanovsky SM
The Journal of biological chemistry 2021 Nov;297(5):101289
The Journal of biological chemistry 2021 Nov;297(5):101289
The PHLPP1 N-Terminal Extension is a Mitotic Cdk1 Substrate and Controls an Interactome Switch.
Kawashima AT, Wong C, Lordén G, King CC, Lara-Gonzalez P, Desai A, Gingras AC, Newton AC
Molecular and cellular biology 2021 Jan 4;
Molecular and cellular biology 2021 Jan 4;
CUL4B negatively regulates Toll-like receptor-triggered proinflammatory responses by repressing Pten transcription.
Song Y, Li P, Qin L, Xu Z, Jiang B, Ma C, Shao C, Gong Y
Cellular & molecular immunology 2021 Feb;18(2):339-349
Cellular & molecular immunology 2021 Feb;18(2):339-349
Inhibition of PHLPP1/2 phosphatases rescues pancreatic β-cells in diabetes.
Lupse B, Annamalai K, Ibrahim H, Kaur S, Geravandi S, Sarma B, Pal A, Awal S, Joshi A, Rafizadeh S, Madduri MK, Khazaei M, Liu H, Yuan T, He W, Gorrepati KDD, Azizi Z, Qi Q, Ye K, Oberholzer J, Maedler K, Ardestani A
Cell reports 2021 Aug 3;36(5):109490
Cell reports 2021 Aug 3;36(5):109490
Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.
He X, Li M, Yu H, Liu G, Wang N, Yin C, Tu Q, Narla G, Tao Y, Cheng S, Yin H
PLoS biology 2020 Dec;18(12):e3000803
PLoS biology 2020 Dec;18(12):e3000803
Cigarette smoke extract induces airway epithelial cell death via repressing PRMT6/AKT signaling.
Li T, Fanning KV, Nyunoya T, Chen Y, Zou C
Aging 2020 Dec 1;12(23):24301-24317
Aging 2020 Dec 1;12(23):24301-24317
Inhibition of PHLPP1 ameliorates cardiac dysfunction via activation of the PI3K/Akt/mTOR signalling pathway in diabetic cardiomyopathy.
Zhang M, Wang X, Liu M, Liu D, Pan J, Tian J, Jin T, Xu Y, An F
Journal of cellular and molecular medicine 2020 Apr;24(8):4612-4623
Journal of cellular and molecular medicine 2020 Apr;24(8):4612-4623
USP1 deubiquitinates Akt to inhibit PI3K-Akt-FoxO signaling in muscle during prolonged starvation.
Goldbraikh D, Neufeld D, Eid-Mutlak Y, Lasry I, Gilda JE, Parnis A, Cohen S
EMBO reports 2020 Apr 3;21(4):e48791
EMBO reports 2020 Apr 3;21(4):e48791
Phlpp1 is associated with human intervertebral disc degeneration and its deficiency promotes healing after needle puncture injury in mice.
Zhang C, Smith MP, Zhou GK, Lai A, Hoy RC, Mroz V, Torre OM, Laudier DM, Bradley EW, Westendorf JJ, Iatridis JC, Illien-Jünger S
Cell death & disease 2019 Oct 3;10(10):754
Cell death & disease 2019 Oct 3;10(10):754
PHLPP1 counter-regulates STAT1-mediated inflammatory signaling.
Cohen Katsenelson K, Stender JD, Kawashima AT, Lordén G, Uchiyama S, Nizet V, Glass CK, Newton AC
eLife 2019 Aug 13;8
eLife 2019 Aug 13;8
Protein Kinase C Quality Control by Phosphatase PHLPP1 Unveils Loss-of-Function Mechanism in Cancer.
Baffi TR, Van AN, Zhao W, Mills GB, Newton AC
Molecular cell 2019 Apr 18;74(2):378-392.e5
Molecular cell 2019 Apr 18;74(2):378-392.e5
PHLPP regulates hexokinase 2-dependent glucose metabolism in colon cancer cells.
Xiong X, Wen YA, Mitov MI, C Oaks M, Miyamoto S, Gao T
Cell death discovery 2017;3:16103
Cell death discovery 2017;3:16103
High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.
Xie Y, Lv D, Wang W, Ye M, Chen X, Yang H
Oncotarget 2017 Aug 29;8(35):59000-59007
Oncotarget 2017 Aug 29;8(35):59000-59007
Pleckstrin Homology (PH) Domain Leucine-rich Repeat Protein Phosphatase Controls Cell Polarity by Negatively Regulating the Activity of Atypical Protein Kinase C.
Xiong X, Li X, Wen YA, Gao T
The Journal of biological chemistry 2016 Nov 25;291(48):25167-25178
The Journal of biological chemistry 2016 Nov 25;291(48):25167-25178
PHLPP negatively regulates cell motility through inhibition of Akt activity and integrin expression in pancreatic cancer cells.
Smith AJ, Wen YA, Stevens PD, Liu J, Wang C, Gao T
Oncotarget 2016 Feb 16;7(7):7801-15
Oncotarget 2016 Feb 16;7(7):7801-15
Loss of PHLPP protects against colitis by inhibiting intestinal epithelial cell apoptosis.
Wen YA, Li X, Goretsky T, Weiss HL, Barrett TA, Gao T
Biochimica et biophysica acta 2015 Oct;1852(10 Pt A):2013-23
Biochimica et biophysica acta 2015 Oct;1852(10 Pt A):2013-23
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse brain tissue were subjected to SDS PAGE followed by western blot with 22789-1-AP(PHLPP antibody) at dilution of 1:1000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using PHLPP antibody 22789-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-PHLPP (IP:22789-1-AP, 5ug; Detection:22789-1-AP 1:1000) with mouse brain tissue lysate 5000ug.
- Sample type
- tissue