AP7756B

antibody from Abgent

Targeting: FKBP1A FKBP-12, FKBP1, FKBP12, FKBP12C, PKC12, PPIASE

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7756B

Provider

Abgent

Proper citation

Abgent Cat#AP7756b, RRID:AB_1967679

Product name

FKBP1A Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of FKBP1A antibody (C-term) (Cat.# AP7756b) in Jurkat cell line lysates (35ug/lane). FKBP1A (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of FKBP1A (arrow) using rabbit polyclonal FKBP1A Antibody (C-term) (Cat. #AP7756b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FKBP1A gene.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

FKBP1A Antibody (C-term) (Cat.# AP7756b) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FKBP1A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

FKBP1A Antibody (C-term) (Cat. #AP7756b) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50