MAB3777

antibody from Novus Biologicals

Targeting: FKBP1A FKBP-12, FKBP1, FKBP12, FKBP12C, PKC12, PPIASE

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3777

Provider

Novus Biologicals

Product name

Rat Monoclonal FKBP12 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat FKBP12 in Western blots. In Western blots, no cross-reactivity with other FKBP family members is observed.

Reactivity

Human, Mouse, Rat

Host

Rat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

An inducible, ligand-independent receptor activator of NF-κB gene to control osteoclast differentiation from monocytic precursors.

Rementer CW, Wu M, Buranaphatthana W, Yang HY, Scatena M, Giachelli CM
PloS one 2013;8(12):e84465

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat FKBP12 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat FKBP12 Monoclonal Antibody (Catalog # MAB3777) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human FKBP12 (Catalog # 3777-FK), recombinant human FKBP12.6, and recombinant human FKBP13 (5 ng/lane) were included. A specific band for FKBP12 was detected at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.