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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [34]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-611 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-611, RRID:AB_10002159
- Product name
- Rabbit Polyclonal Rictor Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NB100-611 is specific for human Rictor protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Regulation of mTOR complex 2 signaling in neurofibromatosis 2-deficient target cell types.
A new cytosolic pathway from a Parkinson disease-associated kinase, BRPK/PINK1: activation of AKT via mTORC2.
Temsirolimus and rituximab in patients with relapsed or refractory mantle cell lymphoma: a phase 2 study.
Rapamycin inhibits cytoskeleton reorganization and cell motility by suppressing RhoA expression and activity.
The mTORC2 complex regulates terminal differentiation of C2C12 myoblasts.
Characterization of Rictor phosphorylation sites reveals direct regulation of mTOR complex 2 by S6K1.
Specific activation of mTORC1 by Rheb G-protein in vitro involves enhanced recruitment of its substrate protein.
The insulin/Akt signaling pathway is targeted by intracellular beta-amyloid.
An ATP-competitive mammalian target of rapamycin inhibitor reveals rapamycin-resistant functions of mTORC1.
TORC-specific phosphorylation of mammalian target of rapamycin (mTOR): phospho-Ser2481 is a marker for intact mTOR signaling complex 2.
Curcumin disrupts the Mammalian target of rapamycin-raptor complex.
Perifosine inhibits mammalian target of rapamycin signaling through facilitating degradation of major components in the mTOR axis and induces autophagy.
Biochemical, cellular, and in vivo activity of novel ATP-competitive and selective inhibitors of the mammalian target of rapamycin.
Increased AKT S473 phosphorylation after mTORC1 inhibition is rictor dependent and does not predict tumor cell response to PI3K/mTOR inhibition.
Enhancing mammalian target of rapamycin (mTOR)-targeted cancer therapy by preventing mTOR/raptor inhibition-initiated, mTOR/rictor-independent Akt activation.
Mitochondrial metabolism modulates differentiation and teratoma formation capacity in mouse embryonic stem cells.
Cytoplasmic and nuclear distribution of the protein complexes mTORC1 and mTORC2: rapamycin triggers dephosphorylation and delocalization of the mTORC2 components rictor and sin1.
Isolation of hyperactive mutants of mammalian target of rapamycin.
Interaction between simian virus 40 large T antigen and insulin receptor substrate 1 is disrupted by the K1 mutation, resulting in the loss of large T antigen-mediated phosphorylation of Akt.
The TSC1-TSC2 complex is required for proper activation of mTOR complex 2.
Akt-dependent regulation of NF-{kappa}B is controlled by mTOR and Raptor in association with IKK.
CD40-induced signaling in human endothelial cells results in mTORC2- and Akt-dependent expression of vascular endothelial growth factor in vitro and in vivo.
CD40-induced signaling in human endothelial cells results in mTORC2- and Akt-dependent expression of vascular endothelial growth factor in vitro and in vivo.
Nuclear forkhead box O1 controls and integrates key signaling pathways in hepatocytes.
Regulation of mammalian target of rapamycin activity in PTEN-inactive prostate cancer cells by I kappa B kinase alpha.
Point mutations in TOR confer Rheb-independent growth in fission yeast and nutrient-independent mammalian TOR signaling in mammalian cells.
mTORC2 activity is elevated in gliomas and promotes growth and cell motility via overexpression of rictor.
Akt inhibitor A-443654 induces rapid Akt Ser-473 phosphorylation independent of mTORC1 inhibition.
The effects of mTOR-Akt interactions on anti-apoptotic signaling in vascular endothelial cells.
The mammalian target of rapamycin (mTOR) pathway regulates mitochondrial oxygen consumption and oxidative capacity.
Multiallelic disruption of the rictor gene in mice reveals that mTOR complex 2 is essential for fetal growth and viability.
SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity.
Chronic ethanol intake impairs insulin signaling in rats by disrupting Akt association with the cell membrane. Role of TRB3 in inhibition of Akt/protein kinase B activation.
mTOR.RICTOR is the Ser473 kinase for Akt/protein kinase B in 3T3-L1 adipocytes.
James MF, Stivison E, Beauchamp R, Han S, Li H, Wallace MR, Gusella JF, Stemmer-Rachamimov AO, Ramesh V
Molecular cancer research : MCR 2012 May;10(5):649-59
Molecular cancer research : MCR 2012 May;10(5):649-59
A new cytosolic pathway from a Parkinson disease-associated kinase, BRPK/PINK1: activation of AKT via mTORC2.
Murata H, Sakaguchi M, Jin Y, Sakaguchi Y, Futami J, Yamada H, Kataoka K, Huh NH
The Journal of biological chemistry 2011 Mar 4;286(9):7182-9
The Journal of biological chemistry 2011 Mar 4;286(9):7182-9
Temsirolimus and rituximab in patients with relapsed or refractory mantle cell lymphoma: a phase 2 study.
Ansell SM, Tang H, Kurtin PJ, Koenig PA, Inwards DJ, Shah K, Ziesmer SC, Feldman AL, Rao R, Gupta M, Erlichman C, Witzig TE
The Lancet. Oncology 2011 Apr;12(4):361-8
The Lancet. Oncology 2011 Apr;12(4):361-8
Rapamycin inhibits cytoskeleton reorganization and cell motility by suppressing RhoA expression and activity.
Liu L, Luo Y, Chen L, Shen T, Xu B, Chen W, Zhou H, Han X, Huang S
The Journal of biological chemistry 2010 Dec 3;285(49):38362-73
The Journal of biological chemistry 2010 Dec 3;285(49):38362-73
The mTORC2 complex regulates terminal differentiation of C2C12 myoblasts.
Shu L, Houghton PJ
Molecular and cellular biology 2009 Sep;29(17):4691-700
Molecular and cellular biology 2009 Sep;29(17):4691-700
Characterization of Rictor phosphorylation sites reveals direct regulation of mTOR complex 2 by S6K1.
Dibble CC, Asara JM, Manning BD
Molecular and cellular biology 2009 Nov;29(21):5657-70
Molecular and cellular biology 2009 Nov;29(21):5657-70
Specific activation of mTORC1 by Rheb G-protein in vitro involves enhanced recruitment of its substrate protein.
Sato T, Nakashima A, Guo L, Tamanoi F
The Journal of biological chemistry 2009 May 8;284(19):12783-91
The Journal of biological chemistry 2009 May 8;284(19):12783-91
The insulin/Akt signaling pathway is targeted by intracellular beta-amyloid.
Lee HK, Kumar P, Fu Q, Rosen KM, Querfurth HW
Molecular biology of the cell 2009 Mar;20(5):1533-44
Molecular biology of the cell 2009 Mar;20(5):1533-44
An ATP-competitive mammalian target of rapamycin inhibitor reveals rapamycin-resistant functions of mTORC1.
Thoreen CC, Kang SA, Chang JW, Liu Q, Zhang J, Gao Y, Reichling LJ, Sim T, Sabatini DM, Gray NS
The Journal of biological chemistry 2009 Mar 20;284(12):8023-32
The Journal of biological chemistry 2009 Mar 20;284(12):8023-32
TORC-specific phosphorylation of mammalian target of rapamycin (mTOR): phospho-Ser2481 is a marker for intact mTOR signaling complex 2.
Copp J, Manning G, Hunter T
Cancer research 2009 Mar 1;69(5):1821-7
Cancer research 2009 Mar 1;69(5):1821-7
Curcumin disrupts the Mammalian target of rapamycin-raptor complex.
Beevers CS, Chen L, Liu L, Luo Y, Webster NJ, Huang S
Cancer research 2009 Feb 1;69(3):1000-8
Cancer research 2009 Feb 1;69(3):1000-8
Perifosine inhibits mammalian target of rapamycin signaling through facilitating degradation of major components in the mTOR axis and induces autophagy.
Fu L, Kim YA, Wang X, Wu X, Yue P, Lonial S, Khuri FR, Sun SY
Cancer research 2009 Dec 1;69(23):8967-76
Cancer research 2009 Dec 1;69(23):8967-76
Biochemical, cellular, and in vivo activity of novel ATP-competitive and selective inhibitors of the mammalian target of rapamycin.
Yu K, Toral-Barza L, Shi C, Zhang WG, Lucas J, Shor B, Kim J, Verheijen J, Curran K, Malwitz DJ, Cole DC, Ellingboe J, Ayral-Kaloustian S, Mansour TS, Gibbons JJ, Abraham RT, Nowak P, Zask A
Cancer research 2009 Aug 1;69(15):6232-40
Cancer research 2009 Aug 1;69(15):6232-40
Increased AKT S473 phosphorylation after mTORC1 inhibition is rictor dependent and does not predict tumor cell response to PI3K/mTOR inhibition.
Breuleux M, Klopfenstein M, Stephan C, Doughty CA, Barys L, Maira SM, Kwiatkowski D, Lane HA
Molecular cancer therapeutics 2009 Apr;8(4):742-53
Molecular cancer therapeutics 2009 Apr;8(4):742-53
Enhancing mammalian target of rapamycin (mTOR)-targeted cancer therapy by preventing mTOR/raptor inhibition-initiated, mTOR/rictor-independent Akt activation.
Wang X, Yue P, Kim YA, Fu H, Khuri FR, Sun SY
Cancer research 2008 Sep 15;68(18):7409-18
Cancer research 2008 Sep 15;68(18):7409-18
Mitochondrial metabolism modulates differentiation and teratoma formation capacity in mouse embryonic stem cells.
Schieke SM, Ma M, Cao L, McCoy JP Jr, Liu C, Hensel NF, Barrett AJ, Boehm M, Finkel T
The Journal of biological chemistry 2008 Oct 17;283(42):28506-12
The Journal of biological chemistry 2008 Oct 17;283(42):28506-12
Cytoplasmic and nuclear distribution of the protein complexes mTORC1 and mTORC2: rapamycin triggers dephosphorylation and delocalization of the mTORC2 components rictor and sin1.
Rosner M, Hengstschläger M
Human molecular genetics 2008 Oct 1;17(19):2934-48
Human molecular genetics 2008 Oct 1;17(19):2934-48
Isolation of hyperactive mutants of mammalian target of rapamycin.
Ohne Y, Takahara T, Hatakeyama R, Matsuzaki T, Noda M, Mizushima N, Maeda T
The Journal of biological chemistry 2008 Nov 14;283(46):31861-70
The Journal of biological chemistry 2008 Nov 14;283(46):31861-70
Interaction between simian virus 40 large T antigen and insulin receptor substrate 1 is disrupted by the K1 mutation, resulting in the loss of large T antigen-mediated phosphorylation of Akt.
Yu Y, Alwine JC
Journal of virology 2008 May;82(9):4521-6
Journal of virology 2008 May;82(9):4521-6
The TSC1-TSC2 complex is required for proper activation of mTOR complex 2.
Huang J, Dibble CC, Matsuzaki M, Manning BD
Molecular and cellular biology 2008 Jun;28(12):4104-15
Molecular and cellular biology 2008 Jun;28(12):4104-15
Akt-dependent regulation of NF-{kappa}B is controlled by mTOR and Raptor in association with IKK.
Dan HC, Cooper MJ, Cogswell PC, Duncan JA, Ting JP, Baldwin AS
Genes & development 2008 Jun 1;22(11):1490-500
Genes & development 2008 Jun 1;22(11):1490-500
CD40-induced signaling in human endothelial cells results in mTORC2- and Akt-dependent expression of vascular endothelial growth factor in vitro and in vivo.
Dormond O, Contreras AG, Meijer E, Datta D, Flynn E, Pal S, Briscoe DM
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8088-95
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8088-95
CD40-induced signaling in human endothelial cells results in mTORC2- and Akt-dependent expression of vascular endothelial growth factor in vitro and in vivo.
Dormond O, Contreras AG, Meijer E, Datta D, Flynn E, Pal S, Briscoe DM
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8088-95
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8088-95
Nuclear forkhead box O1 controls and integrates key signaling pathways in hepatocytes.
Naïmi M, Gautier N, Chaussade C, Valverde AM, Accili D, Van Obberghen E
Endocrinology 2007 May;148(5):2424-34
Endocrinology 2007 May;148(5):2424-34
Regulation of mammalian target of rapamycin activity in PTEN-inactive prostate cancer cells by I kappa B kinase alpha.
Dan HC, Adli M, Baldwin AS
Cancer research 2007 Jul 1;67(13):6263-9
Cancer research 2007 Jul 1;67(13):6263-9
Point mutations in TOR confer Rheb-independent growth in fission yeast and nutrient-independent mammalian TOR signaling in mammalian cells.
Urano J, Sato T, Matsuo T, Otsubo Y, Yamamoto M, Tamanoi F
Proceedings of the National Academy of Sciences of the United States of America 2007 Feb 27;104(9):3514-9
Proceedings of the National Academy of Sciences of the United States of America 2007 Feb 27;104(9):3514-9
mTORC2 activity is elevated in gliomas and promotes growth and cell motility via overexpression of rictor.
Masri J, Bernath A, Martin J, Jo OD, Vartanian R, Funk A, Gera J
Cancer research 2007 Dec 15;67(24):11712-20
Cancer research 2007 Dec 15;67(24):11712-20
Akt inhibitor A-443654 induces rapid Akt Ser-473 phosphorylation independent of mTORC1 inhibition.
Han EK, Leverson JD, McGonigal T, Shah OJ, Woods KW, Hunter T, Giranda VL, Luo Y
Oncogene 2007 Aug 16;26(38):5655-61
Oncogene 2007 Aug 16;26(38):5655-61
The effects of mTOR-Akt interactions on anti-apoptotic signaling in vascular endothelial cells.
Dormond O, Madsen JC, Briscoe DM
The Journal of biological chemistry 2007 Aug 10;282(32):23679-86
The Journal of biological chemistry 2007 Aug 10;282(32):23679-86
The mammalian target of rapamycin (mTOR) pathway regulates mitochondrial oxygen consumption and oxidative capacity.
Schieke SM, Phillips D, McCoy JP Jr, Aponte AM, Shen RF, Balaban RS, Finkel T
The Journal of biological chemistry 2006 Sep 15;281(37):27643-52
The Journal of biological chemistry 2006 Sep 15;281(37):27643-52
Multiallelic disruption of the rictor gene in mice reveals that mTOR complex 2 is essential for fetal growth and viability.
Shiota C, Woo JT, Lindner J, Shelton KD, Magnuson MA
Developmental cell 2006 Oct;11(4):583-9
Developmental cell 2006 Oct;11(4):583-9
SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity.
Jacinto E, Facchinetti V, Liu D, Soto N, Wei S, Jung SY, Huang Q, Qin J, Su B
Cell 2006 Oct 6;127(1):125-37
Cell 2006 Oct 6;127(1):125-37
Chronic ethanol intake impairs insulin signaling in rats by disrupting Akt association with the cell membrane. Role of TRB3 in inhibition of Akt/protein kinase B activation.
He L, Simmen FA, Mehendale HM, Ronis MJ, Badger TM
The Journal of biological chemistry 2006 Apr 21;281(16):11126-34
The Journal of biological chemistry 2006 Apr 21;281(16):11126-34
mTOR.RICTOR is the Ser473 kinase for Akt/protein kinase B in 3T3-L1 adipocytes.
Hresko RC, Mueckler M
The Journal of biological chemistry 2005 Dec 9;280(49):40406-16
The Journal of biological chemistry 2005 Dec 9;280(49):40406-16
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rictor Antibody [NB100-611] - Detection of Human Rictor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody NB100-611 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rictor Antibody [NB100-611] - Detection of Human Rictoron HeLa whole cell lysate using NB100-611. Rictor was also IPed using rabbit anti-Rictor antibodies NB100-612 and another one.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Rictor Antibody [NB100-611] - Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rictor antibody NB100-611 (lot 5) used for IP at 6 ug per reaction. Rictor was also immunoprecipitated by a previous lot of this antibody (lot 4) and rabbit anti-Rictor antibody NB100-612. For blotting immunoprecipitated Rictor, NB100-611 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
