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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2054 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2054, RRID:AB_1579764
- Product name
- RICTOR polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of RICTOR.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of Protor as a novel Rictor-binding component of mTOR complex-2.
SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity.
Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity.
Pearce LR, Huang X, Boudeau J, Pawłowski R, Wullschleger S, Deak M, Ibrahim AF, Gourlay R, Magnuson MA, Alessi DR
The Biochemical journal 2007 Aug 1;405(3):513-22
The Biochemical journal 2007 Aug 1;405(3):513-22
SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity.
Jacinto E, Facchinetti V, Liu D, Soto N, Wei S, Jung SY, Huang Q, Qin J, Su B
Cell 2006 Oct 6;127(1):125-37
Cell 2006 Oct 6;127(1):125-37
Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity.
Yang Q, Inoki K, Ikenoue T, Guan KL
Genes & development 2006 Oct 15;20(20):2820-32
Genes & development 2006 Oct 15;20(20):2820-32
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of RICTOR polyclonal antibody (Cat # PAB2054) in SK-BR-3 cell line lysates (35 ug/lane).RICTOR (arrow) was detected using the purified Polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue reacted with RICTOR polyclonal antibody (Cat # PAB2054) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
