H00253260-D01P

antibody from Abnova Corporation

Targeting: RICTOR AVO3, KIAA1999, MGC39830, PIA

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00253260-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00253260-D01P, RRID:AB_1579765

Product name

RICTOR purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human RICTOR protein.

Antigen sequence

MAAIGRGRSLKNLRVRGRNDSGEENVPLDLTREPS
DNLREILQNVARLQGVSNMRKLGHLNNFTKLLCDI
GHSEEKLGFHYEDIIICLRLALLNEAKEVRAAGLR
ALRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNE
VERTQALRLVRKMITVNASLFPSSVTNSLIAVGND
GLQERDRMVRACIAIICELALQNPEVVALRGGLNT
ILKNVIDCQLSRINEALITTILHLLNHPKTRQYVR
ADVELEVGTLIFFSSFKILL


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RICTOR expression in transfected 293T cell line (H00253260-T02) by RICTOR MaxPab polyclonal antibody.Lane 1: RICTOR transfected lysate(29.80 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between RICTOR and RPS6KB1. HeLa cells were stained with anti-RICTOR rabbit purified polyclonal 1:1200 and anti-RPS6KB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)