Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10313 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10313, RRID:AB_11183252
- Product name
- RICTOR monoclonal antibody, clone 7B3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant RICTOR.
- Isotype
- IgG
- Antibody clone number
- 7B3
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) against HeLa (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue (A) and breast carcinoma (B) using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green) and negative control (purple).
- Validation comment
- Flow Cytometry