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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A300-459A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Rictor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse Rictor by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody (Product # A300-459A; lot A300-459A-4) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Rictor by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody (Product # A300-459A; lot A300-459A-4) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Rictor antibody A300-459A (lot A300-459A-3) used for IP at 6 µg/mg lysate. Rictor was also immunoprecipitated by a previous lot (A300-459A-2) of this antibody. For blotting immunoprecipitated Rictor, A300-459A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse Rictor by western blot. Samples: Whole cell lysate from NIH3T3 (50µg) and TCKM (50µg) cells. Antibodies: Affinity purified rabbit anti-Rictor antibody A300-459A (lot A300-459A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Rictor by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg), 293T (50µg), and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-Rictor antibody A300-459A (lot A300-459A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Rictor by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Rictor (Product # A300-459A; lot 4) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Rictor by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Rictor (Cat. No. A300-459A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rictor antibody (Product # A300-459A; lot A300-459A-4) used for IP at 3 µg per reaction. Rictor was also immunoprecipitated by a previous lot of this antibody (lot A300-459A-3) and rabbit anti-Rictor antibody (Product # A300-458A). For blotting immunoprecipitated Rictor (Product # A300-459A) was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
