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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [2]
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- Product number
- NB100-1534 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1534, RRID:AB_10001187
- Product name
- Goat Polyclonal Rictor Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Combined RNAi-mediated suppression of Rictor and EGFR resulted in complete tumor regression in an orthotopic glioblastoma tumor model.
Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton.
Verreault M, Weppler SA, Stegeman A, Warburton C, Strutt D, Masin D, Bally MB
PloS one 2013;8(3):e59597
PloS one 2013;8(3):e59597
Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton.
Sarbassov DD, Ali SM, Kim DH, Guertin DA, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM
Current biology : CB 2004 Jul 27;14(14):1296-302
Current biology : CB 2004 Jul 27;14(14):1296-302
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Rictor Antibody [NB100-1534] - The combined silencing of Rictor and EGFR in vivo results in a complete inhibition of tumor growth. U251Ng2x, U251Rictor, U251EGFR and U251EGFR/Rictor cells were implanted into the right caudate nucleus-putamen of Rag2M mice. Induction of shRNA expression in mice was initiated on day 21 by dissolving 2 mg/mL doxycyline and 5% sucrose in drinking water. On day 49, animals were imaged by Maestro (TM) fluorescence imaging unit expression of tRFP co-expressed with the shRNA sequences. Mice brains were harvested, sectioned and stained for nuclei, Rictor, EGFR and p(473)-AKT and imaged for all markers in addition to tRFP by robotic fluorescence microscopy. No tumor was detected in the U251EGFR/Rictor group. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0059597), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rictor Antibody [NB100-1534] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
