MA5-15681

antibody from Invitrogen Antibodies

Targeting: RICTOR AVO3, KIAA1999, MGC39830, PIA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15681

Provider

Invitrogen Antibodies

Product name

RICTOR Monoclonal Antibody (7B3)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15681 targets RICTOR in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples. The MA5-15681 immunogen is purified recombinant fragment of human RICTOR expressed in E. Coli. MA5-15681 detects RICTOR which has a predicted molecular weight of approximately 192kDa.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

7B3

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Lamellipodin-RICTOR Signaling Mediates Glioblastoma Cell Invasion and Radiosensitivity Downstream of EGFR.

Moritz S, Krause M, Schlatter J, Cordes N, Vehlow A
Cancers 2021 Oct 24;13(21)

---

Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency.

Mathieu J, Detraux D, Kuppers D, Wang Y, Cavanaugh C, Sidhu S, Levy S, Robitaille AM, Ferreccio A, Bottorff T, McAlister A, Somasundaram L, Artoni F, Battle S, Hawkins RD, Moon RT, Ware CB, Paddison PJ, Ruohola-Baker H
Nature communications 2019 Feb 7;10(1):632

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RICTOR using RICTOR monoclonal antibody (Product # MA5-15681) in HeLa (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NIH/3T3 cells using RICTOR monoclonal antibody (Product # MA5-15681) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Rictor (magenta) in Hela cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 2% BSA in PBS for 60 minutes at room temperature. Cells were stained with a Rictor monoclonal antibody (Product # MA5-15681) at a dilution of 1:200 in staining buffer for 3 hour at room temperature, and then incubated with a Goat anti-mouse IgG Secondary Antibody, Alexa Fluor 647 conjugate (Product # A32728) at a dilution of 1:1000 for 1 hour at room temperature (magenta). Nuclei (blue) were counterstained with DAPI dye. Images were taken at 100X magnification. Data courtesy of Thermo Scientific KOL Program.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NIH/3T3 cells using RICTOR monoclonal antibody (Product # MA5-15681) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR monoclonal antibody (Product # MA5-15681) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using RICTOR monoclonal antibody (Product # MA5-15681) (green) and negative control (purple).