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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-59547 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IVD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LLPVDDAING LSEEQRQLRQ TMAKFLQEHL APKAQEIDRS NEFKNLREFW KQLGNLGVLG ITAPVQYGGS GLGYLEHVLV MEEISRASGA VGLSYGAHSN LCINQLVRNG NE Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 88%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Mild inborn errors of metabolism in commonly used inbred mouse strains.
Leandro J, Violante S, Argmann CA, Hagen J, Dodatko T, Bender A, Zhang W, Williams EG, Bachmann AM, Auwerx J, Yu C, Houten SM
Molecular genetics and metabolism 2019 Apr;126(4):388-396
Molecular genetics and metabolism 2019 Apr;126(4):388-396
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IVD in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using an IVD Polyclonal Antibody (Product # PA5-59547).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of IVD in human cell line U-2 OS shows positivity in mitochondria & nucleus but excluded from the nucleoli. Samples were probed using an IVD Polyclonal Antibody (Product # PA5-59547).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IVD in human thyroid gland and skeletal muscle tissues using IVD Polyclonal Antibody (Product # PA5-59547). Corresponding IVD RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IVD in human thyroid gland using IVD Polyclonal Antibody (Product # PA5-59547) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IVD in human skeletal muscle using IVD Polyclonal Antibody (Product # PA5-59547) shows low expression as expected.
