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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-22342 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IVD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, Jurkat, mouse brain, PC-12. Predicted reactivity: Mouse (90%), Rat (90%), Pig (91%), Chicken (85%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Ataxin-2 (Atxn2)-Knock-Out Mice Show Branched Chain Amino Acids and Fatty Acids Pathway Alterations.
Meierhofer D, Halbach M, Şen NE, Gispert S, Auburger G
Molecular & cellular proteomics : MCP 2016 May;15(5):1728-39
Molecular & cellular proteomics : MCP 2016 May;15(5):1728-39
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IVD Polyclonal Antibody (Product # PA5-22342). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. IVD Polyclonal Antibody (Product # PA5-22342) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IVD Polyclonal Antibody detects IVD protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.10 % SDS-PAGE. IVD Polyclonal Antibody (Product # PA5-22342) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IVD Polyclonal Antibody (Product # PA5-22342). Sample (30 µg of whole cell lysate). Lane A: Hela. Lane B: JurKat. 10% SDS PAGE. IVD Polyclonal Antibody (Product # PA5-22342) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IVD in methanol-fixed HeLa cells using an IVD polyclonal antibody (Product # PA5-22342) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using IVD (Product # PA5-22342) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
