PA5-109241

antibody from Invitrogen Antibodies

Targeting: TLE6 FLJ14009, GRG6

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109241

Provider

Invitrogen Antibodies

Product name

TLE6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Control: Mouse ovary tissue lysate, HepG2, A431, A549, SK-Br-3, mouse small intestine tissue, Daudi.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Tle6 in SK-Br-3 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Tle6 polyclonal antibody (Product # PA5-109241), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Tle6 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Tle6 polyclonal antibody (Product # PA5-109241), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Tle6 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Tle6 polyclonal antibody (Product # PA5-109241), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Tle6 in SK-Br-3 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Tle6 polyclonal antibody (Product # PA5-109241), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Tle6 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Tle6 polyclonal antibody (Product # PA5-109241), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Tle6 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Tle6 polyclonal antibody (Product # PA5-109241), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Tle6 in paraffin-embedded mouse small intestine tissue. Samples were incubated with Tle6 polyclonal antibody (Product # PA5-109241), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Tle6 in Daudi cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Tle6 polyclonal antibody (Product # PA5-109241) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Tle6 in Daudi cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Tle6 polyclonal antibody (Product # PA5-109241) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.