Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN500268 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Mediator Complex Subunit 28 (MED28) (Center) antibody
- Antibody type
- Polyclonal
- Antigen
- MED28 antibody was raised against a 17 amino acid peptide from near the center of human MED28.
- Description
- Peptide affinity chromatography
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- Center
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 2 - 8°C for up to one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Identification of host proteins required for HIV infection through a functional genomic screen.
Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation.
Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2.
A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
Brass AL, Dykxhoorn DM, Benita Y, Yan N, Engelman A, Xavier RJ, Lieberman J, Elledge SJ
Science (New York, N.Y.) 2008 Feb 15;319(5865):921-6
Science (New York, N.Y.) 2008 Feb 15;319(5865):921-6
Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation.
Beyer KS, Beauchamp RL, Lee MF, Gusella JF, Näär AM, Ramesh V
The Journal of biological chemistry 2007 Nov 2;282(44):32152-7
The Journal of biological chemistry 2007 Nov 2;282(44):32152-7
Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2.
Wiederhold T, Lee MF, James M, Neujahr R, Smith N, Murthy A, Hartwig J, Gusella JF, Ramesh V
Oncogene 2004 Nov 18;23(54):8815-25
Oncogene 2004 Nov 18;23(54):8815-25
A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC
Molecular cell 2004 Jun 4;14(5):685-91
Molecular cell 2004 Jun 4;14(5):685-91
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of MED28 in human brain tissue lysate with AP30555PU-N at 1 ?g/ml in (A) the absence and (B) the presence of blocking peptide.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry of MED28 in human brain tissue with AP30555PU-N at 2.5 ?g/ml.